Effect of cigarette smoke on the differentiation, mechano-transduction of osteoblast and molecular events affecting bone remodeling
In skeletal muscle, upon smoking a cigarette, the concentrations of cigarette smoke components (nicotine and cotinine) are close to that of whole blood reaching up to 100ng/ml. Several studies have shown the direct toxicity of CS on osteoblasts, however, its long term effect on the differentiation and mechanotransduction of osteoblasts is still unclear. The mechano-sensitivity of bone is regulated by microtubule based primary cilium which has recently been shown critical in osteoblast differentiation and bone turnover. This study aims at investigating the implications of physiological concentrations of cigarette smoke extract on osteoblast differentiation and the integrity of primary cilia and the underlying molecular mechanisms and cross-talks of key players (BMPs, Wnt, TGF-beta, Hedgehog signaling).
- Dr. sc. hum. Sabrina Ehnert
- Romina Aspera (PhD student)
- Sandra Baumüller (Dr. med. cand.)
- Julius Strobel (Dr. med. cand.)
- Daniel Heid (Dr. med. cand.)
- Influence of oxidative stress and TGF-β induced by cigarette smoking on mechanotransduction of primary cilia in human osteoblast.
- Molecular mechanism underlying the effect of cigarette smoke extract on osteoblasts and its implications in bone remodeling.
- Effect of E-cigarette on osteoblast differentiation and bone remodeling- an alternative to conventional cigarettes?
- Aspera-Werz RH, Ehnert S, Flesch I, Bahrs C, Nüssler AK, S. Kumar V (2016). Cigarette smoke extract exposure affects primary cilia during osteogenic differentiation of SCP-1 cells in vitro. Abstract: 20. Chirurgische Forschungstage Magdeburg).
- Ehnert S, Döbele S, Braun KF, Burkhardt B, Hofmann V, Hausmann M, Egaña JT, Stöckle U, Freude T, Nussler AK (2012). N-Acetylcyteine and flavonoid rich diet: The protective effect of 15 different antioxidants on cigarette smoke-damaged primary human osteoblasts. Advances in Bioscience and Biotechnology / 3 / 1129-39.
- Ehnert S, Braun KF, Buchholz A, Freude T, Egaña JT, Schenck TL, Schyschka L, Neumaier M, Döbele S, Stöckle U, Nussler AK (2012). Diallyl-disulfide is the effective ingredient of garlic oil that protects primary human osteoblasts from damage due to cigarette smoke. Food Chem / 132(2) / 724 -9. IF = 3,33.
- Holzer N, Braun KF, Ehnert S, Buchholz A, Schyschka L, Freude T, Neumaier M, Stöckle U, Nussler AK (2011). Green tea extract and catechins protect human osteoblasts exposed to cigarette smoke through activation of the HO-1. Langenbecks Arch Surg / 397(3) / 467-74. IF = 1,81.
- Braun KF, Ehnert S, Freude T, Egaña JT, Schenck TL, Buchholz A, Schmitt A, Siebenlist S, Schyschka L, Neumaier M, Stöckle U, Nussler AK (2011). Quercetin protects primary human osteoblasts exposed to cigarette smoke through activation of the anti-oxidative enzymes HO-1 and SOD-1. IF = 1,73.
LeiterProf. Dr. Andreas Nüssler
Siegfried Weller Institut für Unfallmedizinische Forschung